A Hollow Sphere Soft Lithography Approach for Long-Term Hanging Drop Methods
نویسندگان
چکیده
منابع مشابه
A hollow sphere soft lithography approach for long-term hanging drop methods.
In conventional hanging drop (HD) methods, embryonic stem cell aggregates or embryoid bodies (EBs) are often maintained in small inverted droplets. Gravity limits the volumes of these droplets to less than 50 microL, and hence such cell cultures can only be sustained for a few days without frequent media changes. Here we present a new approach to performing long-term HD methods (10-15 days) tha...
متن کاملHanging Drop Tissue Culture
The hanging drop tissue culture is a technique utilized in embryology [4] and other fields to allow growth that would otherwise be restricted by the flat plane of culture dishes and also to minimize the surface area to volume ratio, slowing evaporation. The classic hanging drop culture is a small drop of liquid, such as plasma or some other media allowing tissue growth, suspended from an invert...
متن کاملCOVID-19 Intervention Scenarios for a Long-term Disease Management
Background The first outbreak of coronavirus disease 2019 (COVID-19) was successfully restrained in many countries around the world by means of a severe lockdown. Now, we are entering the second phase of the pandemics in which the spread of the virus needs to be contained within the limits that national health systems can cope with. This second phase of the epidemics is expected to last until a...
متن کاملNanopatterning molecularly imprinted polymers by soft lithography: a hierarchical approach.
We use soft lithography to pattern molecularly imprinted polymers (MIPs) at the nanoscale. Patterning occurs via a micro transfer molding process associated with an edge effect. We show using fluorescence microscopy that the nanopatterned synthetic receptors specifically recognize and bind a model target, dansyl-l-phenylalanine. We also demonstrate using AFM a specific swelling of the MIP patte...
متن کاملSoft Lithography for Microfluidics : a Review
Soft lithography has provided a low-expertise route toward micro/nanofabrication and is playing an important role in microfluidics, ranging from simple channel fabrication to the creation of micropatterns onto a surface or within a microfluidic channel. In this review, the materials, methods, and applications of soft lithography for microfluidics are briefly summarized with a particular emphasi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Tissue Engineering Part C: Methods
سال: 2010
ISSN: 1937-3384,1937-3392
DOI: 10.1089/ten.tec.2009.0248